DEPC treatment of ethanol

Michael L. Sullivan mlsulliv at facstaff.wisc.edu
Mon Nov 13 09:41:18 EST 2000


>NO
>Same as you dont DEPC treat other solutions you make up eg EDTA
>etc........you use DEPC treated water only as it breaks specific chemical
>bonds ie heat treatment of some chemical solutions for use inn RNA
>purification.
>

DEPC CAN be used to treat some solutions, just not those made with
amine-containing compounds, like TRIS.  However, LiCl, NaOAc solutions and
the like can be DEPC treated, although such precautions are often not
needed if care is taken, even for water.  As for the specific question
regarding treatment of EtOH-- just use an unopened bottle and use
DEPC-treated (or other RNAse free source) water to make the 70%.

Mike

Michael L. Sullivan, Ph.D

U.S. Dairy Forage Research Center
1925 Linden Drive West
Madison WI, 53706

(608) 264-5144 Phone
(608) 264-5147 Fax


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