peptide biotinylation question

Student mucineer at
Tue Nov 14 05:46:56 EST 2000

I need to biotinylate two 10-amino acid peptides with NHS-X-Biotin. My
problem is this: how can I effectively remove all the unreacted free biotin
ester after the biotinylation reaction? I can't use the normal desalting
methods because the biotinylated peptide is still too small.

Some people suggested using dialysis but are there any dialysis membranes
that have such a small limit? If so, can someone suggest a place where I can
get it in the UK?

Any other suggestions will be appreciated. Thanks.

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