Converting micrograms to moles for IgG

Student mucineer at iname.com
Tue Nov 14 06:16:41 EST 2000


I thought 150kDa means approximately 150,000grams?

I am confused. So can someone confirm this is right:

1 mole of a protein means there is 6.02x10E23 molecules of that protein. and
for an IgG, 150,000grams of the antibody will contain 6.02E23 IgG molecule
which is 1 mole of IgG?



> No, 1 mole is about 150,000 grams.
>
> Rich Miller
>
> clarosa wrote:
>
> > yes 1 mole is about 150 kDA..given that the size is an estimate.
> >
> > Student wrote:
> >
> > > This may sound a very silly question to many of you. Whilst I know how
to
> > > convert mass to moles for small molecules, I have never done it for
large
> > > proteins. For an IgG1 molecule, I know the size is approximately
150kDa.
> > > Does this mean that 1 mole = 150,000grams? (It would be rather tedious
to
> > > add up all the molecular weights of the amino acids to get the molar
mass!)
> > >
> > > Thanks, and excuse my ignorance!
>







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