Converting micrograms to moles for IgG

Mike Clark mrc7 at cam.ac.uk
Tue Nov 14 06:59:46 EST 2000


In article <8uqjmh$9ib$1 at nnrp1.deja.com>,
<URL:mailto:engelbert_buxbaum4 at my-deja.com> wrote:
> In article <8uobvk$d2j$1 at oyez.ccc.nottingham.ac.uk>,
>   "Student" <mucineer at iname.com> wrote:
> >  (It would be rather tedious to
> > add up all the molecular weights of the amino acids to get the molar
> mass!)
> 
> 
> Once a protein is sequenced, there are computer programs that handle the
> maths for you. Antheprot and similar utilities will calculate how many
> times each amino acid occurs in the protein, its molecular weight, pI,
> molar absorbance coeficient at 280 nm, hydropathy profile, prediction of
> antigenic segments and secondary structure and a lot more. Check the
> biology section at SIMTEL or HENSA, if you are interested in such
> utilities, many of which are freeware. Some of them are also run on the
> net, Pedro's Molecular Biology Homepage
> http://www.biophys.uni-duesseldorf.de/bionet/research_tools.html will
> point you to these resources.
> 
> 

Unfortunately many proteins have post-translational modifications so that a
precise measurement of the Mr predicted from the DNA sequence will not be
correct. Immunoglobulin has N-linked and O-linked glycosylation depending
upon the class and subclass.


Mike                            <URL:http://www.path.cam.ac.uk/~mrc7/>
-- 
 o/ \\    //            ||  ,_ o   M.R. Clark, PhD. Division of Immunology
<\__,\\  //   __o       || /  /\,  Cambridge University, Dept. Pathology
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