DEPC treatment of ethanol

Michael L. Sullivan mlsulliv at facstaff.wisc.edu
Tue Nov 14 09:52:31 EST 2000


>What happens when you try to DEPC treat amine-containing solutions fx. TRIS?
>

Well, the DEPC simply reacts with the excess of amine groups in solution.
Consequently, the DEPC is not available to react with amine groups in
proteins, like RNase.  So in this case, it's not a matter of something bad
happening, just a case of wasting money on a treatment that would be
ineffective.

Mike

Michael L. Sullivan, Ph.D

U.S. Dairy Forage Research Center
1925 Linden Drive West
Madison WI, 53706

(608) 264-5144 Phone
(608) 264-5147 Fax


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