Converting micrograms to moles for IgG
clarosa at biocomp.unl.edu
Tue Nov 14 11:14:08 EST 2000
oops 1 mole = about 1.5 E5 grams... sorry.
> I thought 150kDa means approximately 150,000grams?
> I am confused. So can someone confirm this is right:
> 1 mole of a protein means there is 6.02x10E23 molecules of that protein. and
> for an IgG, 150,000grams of the antibody will contain 6.02E23 IgG molecule
> which is 1 mole of IgG?
> > No, 1 mole is about 150,000 grams.
> > Rich Miller
> > clarosa wrote:
> > > yes 1 mole is about 150 kDA..given that the size is an estimate.
> > >
> > > Student wrote:
> > >
> > > > This may sound a very silly question to many of you. Whilst I know how
> > > > convert mass to moles for small molecules, I have never done it for
> > > > proteins. For an IgG1 molecule, I know the size is approximately
> > > > Does this mean that 1 mole = 150,000grams? (It would be rather tedious
> > > > add up all the molecular weights of the amino acids to get the molar
> > > >
> > > > Thanks, and excuse my ignorance!
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