2D PAGE - reproducibility

Pier Carlo Montecucchi pcmontecucchi at compuserve.com
Wed Nov 15 09:51:45 EST 2000


Dear Gerhard:
I will appreciate if you could read my post referred to M.I.E.R. and its
application for protein purification.
Literature is available upon request.
Best regards,
Pier Carlo Montecucchi
Montegen


"Gerhard Schwall" <gschwall at iuct.fhg.de> wrote in message
news:3A12A0E7.C355DEDA at iuct.fhg.de...
> Currently I am doing 2D gels using purchases IPG strips in a Protean IEF
> camber from Biorad.
> I have tremendos variations in my results, even when I run several
> strips with exactly the same sample in a single run.
> A few gels look absolutely ok and others completely unfocused.
> I tried already different things like passive/aktiv rehydration,
> with/without electrode wicks etc. The result is always the same:
> Some gels are totally allright, others are completely unfocused. All
> strips a treated exactly the same!
> I am wondering if anybody had the same problems or might have a
> suggestion what might cause the trouble?
> Regards
> Gerhard
>
>
>
>







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