2D PAGE - reproducibility
dbell at qnis.net
Wed Nov 15 11:19:25 EST 2000
What is M.I.E.R.?
> Dear Gerhard:
> I will appreciate if you could read my post referred to M.I.E.R. and its
> application for protein purification.
> Literature is available upon request.
> Best regards,
> Pier Carlo Montecucchi
> "Gerhard Schwall" <gschwall at iuct.fhg.de> wrote in message
> news:3A12A0E7.C355DEDA at iuct.fhg.de...
> > Currently I am doing 2D gels using purchases IPG strips in a Protean
> > camber from Biorad.
> > I have tremendos variations in my results, even when I run several
> > strips with exactly the same sample in a single run.
> > A few gels look absolutely ok and others completely unfocused.
> > I tried already different things like passive/aktiv rehydration,
> > with/without electrode wicks etc. The result is always the same:
> > Some gels are totally allright, others are completely unfocused. All
> > strips a treated exactly the same!
> > I am wondering if anybody had the same problems or might have a
> > suggestion what might cause the trouble?
> > Regards
> > Gerhard
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