G418 vs. neomycin in selection of transfected culture cells

Dima Klenchin klenchin at REMOVE_TO_REPLY.facstaff.wisc.edu
Thu Nov 16 10:38:42 EST 2000


Christoph Grunau <Christoph.Grunau at igh.cnrs.fr> wrote:
:Dear all,
:
:does anybody knows the advantage of using G418 and not neomycin for the
:selection of (human) culture cells after transfection with a vector
:that contains the neomycin resistant gene?
:

The main advantage is that G-418 works while neomycin does not
with eukariots. With bacteria, there is no difference. Neomycin
does not bind to eukariotic ribosomes - that is why it is used to
treat humans. No so with geniticin (G-418).

        - Dima






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