High molecular weight smear on Western
spshah at stanford.edu
Thu Nov 16 16:02:09 EST 2000
did you check the placebo sera (blank)..if at all it is a problem, you
should expect similar smear..
"Kevin A. Morano" wrote:
> I am experiencing a problem with a new antibody being generated in
> rabbits from a KLH-peptide immunogen. The band of interest is there,
> but I have a dark smear in the upper portion of the gel. I have seen
> this before with another polyclonal made by someone else - does anyone
> know what it might be, and if there are easy ways to eliminate it? It
> doesn't occur when I blot with secondary Ab-HRP alone, so I do know it's
> the new serum. I am using 8% gels and ECL reagents. Any ideas welcomed.
More information about the Methods