High molecular weight smear on Western

Savita Shah spshah at stanford.edu
Thu Nov 16 16:02:09 EST 2000


did you check the placebo sera (blank)..if at all it is a problem, you
should expect  similar smear.. 


"Kevin A. Morano" wrote:
> 
> I am experiencing  a problem with a new antibody being generated in
> rabbits from a KLH-peptide immunogen.  The band of interest is there,
> but I have a dark smear in the upper portion of the gel.  I have seen
> this before with another polyclonal made by someone else - does anyone
> know what it might be, and if there are easy ways to eliminate it?  It
> doesn't occur when I blot with secondary Ab-HRP alone, so I do know it's
> the new serum.  I am using 8% gels and ECL reagents.  Any ideas welcomed.
> 
> Kevin






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