High molecular weight smear on Western

Arnoud van Vliet avvliet at knoware.nl
Thu Nov 16 19:23:28 EST 2000


"Kevin A. Morano" <kevin.a.morano at uth.tmc.edu> wrote:
> rabbits from a KLH-peptide immunogen.  The band of interest is there,
> but I have a dark smear in the upper portion of the gel.  I have seen
> this before with another polyclonal made by someone else - does anyone
> know what it might be, and if there are easy ways to eliminate it?  It

Could it be genomic DNA with histones still attached? Try sonicating, or
shearing through a syringe, and a lot of vortexing of the sample before
loading onto the gel.

hope this helps
Arnoud







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