Peptide concentration assay

Andrew Johnston andrewj at rsp.is
Fri Nov 17 07:57:08 EST 2000


I´m looking for a simple way of measuring the peptide concentration of a
solution. The solution contains  a micro- to millimolar concentrations
of a 8, 9 or 10-mer peptide. These peptides do not contain Trp or Tyr,
therefore diluting in 6M guanidine and measuring th OD at 280 nm will
not work. Is there an alternative to HPLC ?

Regards,

Andy Johnston.



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