Peptide concentration assay

Dima Klenchin klenchin at facstaff.REMOVE_TO_REPLY.wisc.edu
Fri Nov 17 16:39:36 EST 2000


In article <3A152AF1.A9C75827 at rsp.is>, andrewj at rsp.is (Andrew Johnston) wrote:
:I╢m looking for a simple way of measuring the peptide concentration of a
:solution. The solution contains  a micro- to millimolar concentrations
:of a 8, 9 or 10-mer peptide. These peptides do not contain Trp or Tyr,
:therefore diluting in 6M guanidine and measuring th OD at 280 nm will
:not work. Is there an alternative to HPLC ?
:

You can use absorption at 220 nm where peptide bond absorbs.
You can use Lowry or BCA colorimetric assays. 
You can go to some facility and run a simple aminoacid analysis.

        - Dima






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