non-isotopic Northern

Susanne Rohrer "srohrer(removethis)" at
Mon Nov 20 06:52:33 EST 2000

here you can retrieve all the old posts. The fact that you are getting
rRNA bands is a "good" sign, meaningyour message is expresssed, but
maybe your RNA is slightly degraded. This is a problem especially when
you are working with a less abundant message.
We have found you need to work fast, and don't freeze-thaw your RNA too
often. Preferably load it onto the gel the same session you isolate it.


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