96-well pcr sealing
pshinn at mail1.sas.upenn.edu
Mon Nov 20 18:30:43 EST 2000
: I have tried tape, sealing mate, non of them work. I am sure you have
: use 96-well PCR. Could you tell me what kind of machine, plate sealing
: method you used. What could be my problem?
I've been using the same rubber sealing mats from Perkin-Elmer for
over 3 years. The smooth rounded side goes down into the plate and the
pointy side sticks up against the bottom of the lid. There isn't a part
number on them but when you order a pack of PCR strip tubes from PE, you
get one of these mats as well as two strip tube trays. I mainly use
them for cycle sequencing (but I'm doing that in 384 now with PE sealing
tape) but on occassion I will do 96 PCR reactions. I usually don't wash
the mats after cycle sequencing but for PCR, I'll rinse them in a light
bleach solution followed by 70% and then let them dry. These mats have
been the best sealers for me so far.
The mat+96well plate fit into 96well MJs and PE 9700s with the heated
lid on. No problems yet.
Sequencing Coordinator ,___o
pshinn at neomorph.bio.upenn.edu _-\_<,
Arabidopsis thaliana Genome Center (*)/'(*)
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