random mutagenesis

[Frederik Börnke]

Daniel Schumacher schrieb:
> 
> Hello,
> I hope somebody can help me. I´m looking for a method /protocol to introduce
> mutations randomly in a BAC-Vector.
> I already looked for chemicals that I could just add to the media, which I
> thought would be the easiest way, but wasnt lucky in finding some method.
> Also I thought of uv-light ? By the way the bacteria i use are E.coli.
> Most of the methods I found were using random PCR, which is OK if you only
> have some kb but not with 180 kb.
> So if anybody could give me a hint of what chemical to use and how I would
> be very happy!
> I also heard about mutator-strains which can be used for this kind of thing.
> Does anybody know about them?#
> 
> THX
> 
> Daniel

Hallo Daniel,

Stratagene offers an E. coli mutator strain, XL-I Red. Works well.

Ricky

*******************************************************************

Frederik Boernke
Research Group of  Molecular Plant Physiology
Institute for Plant Genetics and Crop Plant Research (IPK)
Corrensstr. 3
06466 Gatersleben
Tel.  039482 -5 321
Fax. 039482 -5 515
http://www.ipk-gatersleben.de