Cloning LightCycler PCR products

lukasfenner at hotmail.com lukasfenner at hotmail.com
Tue Nov 21 09:59:38 EST 2000


Hi all:

Does anyone has experience with cloning LightCycler PCR products? We
have LightCycler real time PCR from Roche with HotStart Taq-Polymerase.
For cloning we use the TOPO TA Cloning Kit from Invitrogen or the
PCR-Script Amp Cloning Kit from Stratagene?

Do you gel isolate it?
Do you add an additional cycling with ThermoCycler (PE) after the
LightCycler?
Do you use special purification steps?
Or do you only use ThermoCycler PCR products?

Thank you and

Cheers,
Lukas Fenner


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