Oligo's as northern probes

Savita Shah spshah at stanford.edu
Tue Nov 21 14:19:46 EST 2000


We have used 15mers in the past ((cac)5 and
(GATA)4) and as far as I remember.. they worked
fine 


Jonathan Howarth wrote:
> 
> Hello everyone,
> 
> Does anyone know the minimum bases generally required when designing
> good specific oligonucleotide probes for northern blotting?
> 
> Hope anyone with experience of this can give me some idea.
> 
> Thanks in advance,
> 
> Jonathan
> 
> """""""""""""""""""""""""""""""""""""""
> Jonathan R. Howarth
> Biochemistry and Physiology,
> IACR-Rothamsted,
> West Common,
> Harpenden,
> Hertfordshire.
> AL5 2JQ
> 
> """""""""""""""""""""""""""""""""""""""






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