Soluble expression

Kevin Brady rcxkb at unix.ccc.nottingham.ac.uk
Wed Nov 22 02:11:48 EST 2000


Hi there,
You could always try refolding from solubilisation of inclusion bodies,
which can often provide reasonable yields.
Can be done either by dialysis or drop-wise dilutions, both standard.
Remember to include something like DTT in your dialysis/dilution buffers
to promote correct disulfide formation.
Cheers
Kevin

--
Kevin Brady
Lab B54
Organic Chemistry Section
School of Chemistry
University of Nottingham
Nottingham
NG7 2RD
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