Calculate to Nuecleic Acid on BeckMan DU-64
Dr. Duncan Clark
Duncan at nospam.demon.co.uk
Wed Nov 22 04:48:24 EST 2000
In article <Pine.SO4.4.05.10011211341060.2893-100000 at imb4127>, the
eminent Monthop Champaruang at BIOSCI/MRC Human Genome Mapping Project
Resource Centre wrote
> Thank you very much for any comments.
Why not simply measure OD 260 and OD 280 and work out the 260:280 ratio
which for pure DNA should be between 1.8 and 2:1.
Assuming that is OK then 1 OD260 is 50ug dsDNA or 33ug ssDNA.
Now to make sure you are not also reading nucleotides or RNA in the
prep. I would run an aliquot (plus serial dilutions versus a serially
diluted standard) on an agarose gel and verify that your 1ug really is
The problem with being on the cutting edge is that you occasionally get
sliced from time to time....
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