Calculate to Nuecleic Acid on BeckMan DU-64

Dr. Duncan Clark Duncan at nospam.demon.co.uk
Wed Nov 22 04:48:24 EST 2000


In article <Pine.SO4.4.05.10011211341060.2893-100000 at imb4127>, the
eminent Monthop Champaruang at BIOSCI/MRC Human Genome Mapping Project
Resource Centre wrote
>  Thank you very much for any comments.

Why not simply measure OD 260 and OD 280 and work out the 260:280 ratio
which for pure DNA should be between 1.8 and 2:1.

Assuming that is OK then 1 OD260 is 50ug dsDNA or 33ug ssDNA.

Now to make sure you are not also reading nucleotides or RNA in the
prep. I would run an aliquot (plus serial dilutions versus a serially
diluted standard) on an agarose gel and verify that your 1ug really is
1ug etc.

Duncan 

 
-- 
The problem with being on the cutting edge is that you occasionally get 
sliced from time to time....

Duncan Clark
GeneSys Ltd.
Tel: +44(0)1252376288
FAX: +44(0)8701640382
http://www.dnamp.com
http://www.genesys.demon.co.uk






More information about the Methods mailing list