isolating 197bp fragment

Robert Mihalek rmihalek at partners.org
Wed Nov 22 09:56:50 EST 2000


I need to isolate a 197bp dsDNA fragment in sufficient quantities for
making a radioactive probe. I have the fragment subcloned in a plasmid
and can easily isolate it from the plasmid with an EcoR I digest.

Most kits for gel purifying dsDNA seem to have a cut-off at around
200bp. I'm wondering if anyone has some tips on how to best proceed
with isolating this fragment? 

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Bob Mihalek  rmihalek at FORMULA1.partners.org
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