isolating 197bp fragment

[Robert Mihalek]

I need to isolate a 197bp dsDNA fragment in sufficient quantities for
making a radioactive probe. I have the fragment subcloned in a plasmid
and can easily isolate it from the plasmid with an EcoR I digest.

Most kits for gel purifying dsDNA seem to have a cut-off at around
200bp. I'm wondering if anyone has some tips on how to best proceed
with isolating this fragment? 

_______________________
Bob Mihalek  rmihalek at FORMULA1.partners.org
remove name of race series to reply
_______________________
Views, comments, etc. are not representative of MGH or its affiliates
IGNORE