liver RNA

Bertrand Collet b.collet at abdn.ac.uk
Thu Nov 23 04:48:34 EST 2000


Hi,
I frequently extract RNA from fish livers using RNAzol and sometime
TRIZOL. I homogeneise the frozen sample (approx 500 mg liver in 1 ml
RNAZOL or TRIZOL, stored at -70 deg) by sonication, and then extract
according to the manufacturer.
It gives high amounts of good quality total RNA (260/280 > 1.8).
Hope this helps,
Bertrand

Stefan Kirov wrote:

> Has anyone experience in RNA isolation from liver?

--
Dr Bertrand Collet
University of Aberdeen
Department of Zoology
Fish Immunology Group
Tillydrone avenue
Aberdeen AB24 2TZ
Scotland
Phone: (+44) (0) 1224 273 796
Fax: (+44) (0) 1224 272 396







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