Oxygen in cell media

Markus Guetlich markus.guetlich at ch.tum.de
Sat Nov 25 06:22:46 EST 2000


We are already using spinner flasks. But we would like to increase the
number of cells during the protein expression phase. Therfore we would like
to install an additional air supply to insure that the cells do not die
because of a lack of oxygen.

Markus



"Y.F.LEUNG" <yfleung at cuhk.edu.hk> schrieb im Newsbeitrag
news:3A1F5C99.5E87CBDA at cuhk.edu.hk...
> Hi,
>
> Have you ever tried the suspension culture?
>
> Y.F.LEUNG
> Markus Guetlich wrote:
>
> > We want to increase the protein yield of our insect cell culture.
> > Therefore we want to oxygenate the culture.
> >  Who knows what kind of air-bubbles are the best ?
> >
> > Dr. Markus Guetlich
> >
> > Technische Universitaet Muenchen
> >
> > Lehrstuhl fuer Organische Chemie und Biochemie
> >
> > Lichhtenbergstr. 4
> >
> > 85747 Garching
> >
> > Germany
> >
> > Tel: +49-89-289-13049
> >
> > FAX: +49-89-289-13363
> >
> > E-mail: markus.guetlich at ch.tum.de
>
> --
> Y.F.LEUNG
> Department of Ophthalmology and Visual Sciences
> Chinese University of Hong Kong
> 3/F Hong Kong Eye Hospital
> 147K Argyle Street, Hong Kong
> Tel: (852) 27623152 Fax: (852) 27159490
> email: leungyukfai at hotmail.com, yfleung at cuhk.edu.hk
> URL: http://i.am/yfleung (My functional genomics home)
>
>







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