Formula to determine coverage
Tom Knight
tk at pasteur.ai.mit.edu
Sun Nov 26 16:16:27 EST 2000
> Quite a qhile I remember seeing a formula that determines the minimum
> number of sub clones required of a certain size that should, within a
> certain confidence limit, give complete coverage of an insert of given
> length. It involved natural logs and things like that.
> Michael Mitchell "Smoke me a kipper,
> User Support I'll be back for breakfast."
> Molecular Biology Software Ace Rimmer, Test Pilot
> +44 (0)171 269 3115 BBC-TV Red Dwarf ENFJ
To get a desired probability P of having a section of your genome in
the library, where the library consists of clones each having a
fraction f of the original genome, you need a number of clones
ln (1-P)
N = ------------
ln (1-f)
So, to get a 99% probability ( a common choice )
ln (1-.99)
N = ------------------------------------------------
ln (1 - (<size of insert> / <size of genome>))
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