Soluble expression

Hartley, Jim JHartley at lifetech.com
Mon Nov 27 06:23:01 EST 2000


Andrew Hayhurst published a paper earlier this year dealing with improving
solubility of scFv's in E. coli:

Prot. Express. Purific. 18, 1-10 (2000).  He found amino fusions with
maltose binding protein helped a good deal, whereas carboxy fusions did not.

Jim Hartley

James L. Hartley, Ph.D.
Life Technologies / Invitrogen, Inc.
PO Box 6482
9800 Medical Center Drive
Rockville, MD 20849-6482
Tel. 301-610-8337
Fax 301-610-8371
jhartley at lifetech.com


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