T7/SP6 riboprobe transcription

Jonathan Howarth jonathan.howarth at bbsrc.ac.uk
Mon Nov 27 09:40:26 EST 2000


Hello all,

Can anyone tell me whether I can amplify an insert in pUBS with T7 and
SP6 primers and then use the PCR product for riboprobe transcription
with T7 and SP6 RNA polymerases?

I just wondered whether transcription would be inefficient with the
recognition sequences for the polymerses being right at the ends of the
fragment.

Any advice would be greatly appreciated to jonathan.howarth at bbsrc.ac.uk

Jonathan






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