PEG

Michael Witty mw132 at mole.bio.cam.ac.uk
Mon Nov 27 11:39:45 EST 2000


Thanks Harold, this reference look very interesting.  Mike

On Mon, 27 Nov 2000, H.V.Taylor wrote:

> Michael Witty <mw132 at mole.bio.cam.ac.uk> wrote:
> 
> >I have had the misfortune of having my PAGE gels go bad, presumably due to
> >a new water supply which we think is contaminated with PEG
> >
> >BUT, PEG has no charge (does it?) so how does it make my gels go bad?
> >Mike.
> 
> Dear Mike,
> 
> if you used the PEG contaminated water to make your gels you might
> have created a two phase system as certain concentrations of PEG and
> poly acrylic acid are incompatible with each other (I'd give you some
> references but I'm too lazy to run over to the library;-) ). The
> result would be opaque gels.
> If you used the water for your samples see: Odom, O.W. et al., Anal.
> Biochem. 245, 249-252 (1997) "The Effect of PEG 8000 on Protein
> Mobility in SDS PAGE and ..."
> 
> Hope this helped,
> 
> Harold
> 
> 
> 
> 






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