Primers reassemble
Jie Sun
jiesun65 at hotmail.com
Mon Nov 27 15:02:48 EST 2000
Hi, there,
I am trying to reassemble my 20 68bp overlapping primers, the first round
PCR looks normal, I got smearing bands from 2 kb to 500bp (my PCR product
will be 1.4 kb), but I got either smearing or short PCR bands on my second
PCR. Any suggestions?
Many thanks!
Jie
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