How big a PCR product can be TA cloned?

Kimberley Snowden ksnowden at
Mon Nov 27 23:09:04 EST 2000

I have cloned a 10 kb PCR product using Promega's pGEM-T kit. It seemed to work
without any problems once I had a good yield of the PCR product. So I see no
reason why 4-5 kb would cause any problems.


Ali El-Salanti wrote:

> What is the biggest PCR product that people has cloned? Is 4-5 kb possible?
> I use Invitrogens TA TOPO cloning kit.

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