How big a PCR product can be TA cloned?
ksnowden at hort.cri.nz
Mon Nov 27 23:09:04 EST 2000
I have cloned a 10 kb PCR product using Promega's pGEM-T kit. It seemed to work
without any problems once I had a good yield of the PCR product. So I see no
reason why 4-5 kb would cause any problems.
Ali El-Salanti wrote:
> What is the biggest PCR product that people has cloned? Is 4-5 kb possible?
> I use Invitrogens TA TOPO cloning kit.
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