How big a PCR product can be TA cloned?

[Kimberley Snowden]

I have cloned a 10 kb PCR product using Promega's pGEM-T kit. It seemed to work
without any problems once I had a good yield of the PCR product. So I see no
reason why 4-5 kb would cause any problems.

cheers
Kim



Ali El-Salanti wrote:

> What is the biggest PCR product that people has cloned? Is 4-5 kb possible?
> I use Invitrogens TA TOPO cloning kit.