What happens when a blot is over baked?

Nick Theodorakis nicholas_theodorakis at urmc.rochester.edu
Wed Nov 29 12:23:05 EST 2000


In article <JIEHICBJDMGFLNGJIBDMAECOCBAA.alansmith at students.wisc.edu>,
  alansmith at students.wisc.edu ("Alan Smith") wrote:
> Hello,
>
> What happens when you bake a northern blot for 22 hours instead of two hours
> at 80 C?  Should I throw the thing out or should I use it?
>

I believe the worry is that, if you are using nitrocellulose, it might
catch on fire if you leave it in the oven too long.

Nick


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Nick Theodorakis
nicholas_theodorakis at urmc.rochester.edu


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