What happens when a blot is over baked?
David L Haviland
David.L.Haviland at uth.tmc.edu
Wed Nov 29 12:30:23 EST 2000
Alan Smith wrote:
> What happens when you bake a northern blot for 22 hours instead of two hours
> at 80 C? Should I throw the thing out or should I use it?
If it is legit nitrocellulose you are poodle-screwed. It will crumble under
stress. Rerun the gel and blot procedure. If it is charged nylon it will
probably be fine but may not tolerate re-probing.
David L. Haviland, Ph.D., Asst. Prof. Immunology
University of Texas - Houston, H.S.C.
Institute of Molecular Medicine, R907
2121 W. Holcombe Blvd., Houston, TX 77030
Thot for the Day:
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