Single-strand DNA nick plasmid assay query

Patrick Lynch pl229 at medschl.cam.ac.uk
Wed Nov 29 14:59:17 EST 2000


I'm assaying proteins for single-strand DNA nicking activity using
supercoiled plasmids.  If any s/s nicking is present, the nicked plasmid
migrates at a slower rate than the uncut supercoiled plasmid in a 1% agarose
1XTBE gel.  Does the presence of ethidium bromide in the gel during
electrophoresis improve the separation between the uncut and nicked bands?

Thanks

Patrick


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