bacterial expression vector

Johan Burger jtb at maties.sun.ac.za
Thu Nov 30 07:10:24 EST 2000


Hi

We need to express a eukaryotic (plant) gene in bacteria with the aim to
produce antibodies to the protein. Initial attempts (using the Novagen pET
14b vector) were unsuccessful. We expect that our protein might be toxic.

Can anybody recommend a good prokaryotic system to express this protein?

Thanks
Johan Burger







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