Ponceau S staining problem...

Sean Patterson seanpat at fmed2.uncu.edu.ar
Thu Nov 30 08:14:05 EST 2000


>I've tried 2 or 3 different protocols for Ponceau staining of
>nitrocellulose membranes, and can't seem to get my proteins to stain...
>the subsequent westerns are fine, indicating that protein electrophoresis
>and blotting was successful... but all I ever see with Ponceau is the
>tracking dye showing up. This doesn't appear to be a matter of amount of
>proteins, as I've tried putting a lot on the membrane and still nothing.
>I'm using Bio-Rad NC if that is useful info... any suggestions??
>
>Neal
>
>"The most beautiful thing we can experience is the mysterious. It is the
>source of all true art and science"
>            
>-- Albert Einstein
>
>Neal Melvin
>University of Calgary - Faculty of Medicine
>Department of Neuroscience - Neuroscience Research Group (NRG)
>Health Sciences Centre
>3330 Hospital Drive, NW
>Calgary, Alberta, Canada
>T2N 4N1
>
>e-mail: nrmelvin at ucalgary.ca
>Phone: (403) 220-4490 (lab)
>        (403) 220-7035 (office)
>        (403) 283-8731 (fax)
>

Are you blotting with SDS present? I find it interferes substantially 
with the Ponceau staining - but a couple of washes in water (or 
buffer) sort out the problem

Sean


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