What happens when a blot is over baked?

John Dixon jpcd100 at mole.bio.cam.ac.uk
Thu Nov 30 09:30:34 EST 2000

In article <3A24E7DD.2E293110 at uth.tmc.edu>, David L Haviland
<David.L.Haviland at uth.tmc.edu> wrote:

> Alan Smith wrote:
> > Hello,
> >
> > What happens when you bake a northern blot for 22 hours instead of two hours
> > at 80 C?  Should I throw the thing out or should I use it?

On a similar note, does anyone know what happens to blots (Hybond N+)
if these mishaps occur:

1) Left on blotting apparatus for nearly a week (alkali 0.4M NaOH)?

2) Left in cooled to room temp stripping solution (0.1% SDS) after
stripping, for nearly a week?



John Dixon                    Lab 44 (1223) 334131
Wellcome/CRC Institute        Fax 44 (1223) 334089
Cambridge University
United Kingdom CB2 1QR       e-m: jpcd100 at mole.bio.cam.ac.uk

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