What happens when a blot is over baked?

Nick Theodorakis nicholas_theodorakis at urmc.rochester.edu
Thu Nov 30 11:12:24 EST 2000


In article <301120001430349156%jpcd100 at mole.bio.cam.ac.uk>,
  jpcd100 at mole.bio.cam.ac.uk wrote:
> In article <3A24E7DD.2E293110 at uth.tmc.edu>, David L Haviland

[...]

>
> On a similar note, does anyone know what happens to blots (Hybond N+)
> if these mishaps occur:
>
> 1) Left on blotting apparatus for nearly a week (alkali 0.4M NaOH)?
>

Was this a Northern? The RNA is most certainly hydrolyzed by then.

Nick

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_______________________________________________
Nick Theodorakis
nicholas_theodorakis at urmc.rochester.edu


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