bacterial expression vector

Deanne Bell dbell at qnis.net
Thu Nov 30 11:24:48 EST 2000


Is bacteria the only expression system you can use?  can you use yeast?
Are you then going to isolate the protein from the bacteria culture?  can
you isolate the protein from the plant tissue or will you not get enough
protein to use as a antigen? 

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> Hi
> 
> We need to express a eukaryotic (plant) gene in bacteria with the aim to
> produce antibodies to the protein. Initial attempts (using the Novagen
pET
> 14b vector) were unsuccessful. We expect that our protein might be toxic.
> 
> Can anybody recommend a good prokaryotic system to express this protein?
> 
> Thanks
> Johan Burger
> 
> 
> 


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