Ponceau S staining problem...

Louis Hom lhom at OCF.Berkeley.EDU
Thu Nov 30 11:53:34 EST 2000


I second the comment on making sure SDS is gone.  I usually rinse my blots 
a few times in water, then rock for a few minutes in water just to make 
sure the SDS is gone (on my immobilon, the sheeting vs beading of the 
water changes).  I then stain using 0.1% (w/v) Ponceau S in 5% HOAc.
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______________________________________________________________________________
Lou Hom >K'93			     
lhom at ocf.berkeley.edu		
http://www.ocf.berkeley.edu/~lhom/ 	    






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