Ponceau S staining problem...

[Sean Patterson]

>Something I've always wondered... why is a water wash ok?? I mean, isn't
>it important that you keep the pH stable for the proteins on the
>membrane??
>

Since hydrophobic interactions are important in the binding to 
nitrocellulose, the pH isn't critical. In any case, I assume that a 
couple of water wahes will still be buffered from the remaining 
transfer buffer.....

Sean


---