Ponceau S staining problem...

Sean Patterson seanpat at fmed2.uncu.edu.ar
Thu Nov 30 13:38:46 EST 2000


>Something I've always wondered... why is a water wash ok?? I mean, isn't
>it important that you keep the pH stable for the proteins on the
>membrane??
>

Since hydrophobic interactions are important in the binding to 
nitrocellulose, the pH isn't critical. In any case, I assume that a 
couple of water wahes will still be buffered from the remaining 
transfer buffer.....

Sean


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