scFv antibody panning

Josh Neufeld nitrogenase at
Thu Nov 30 20:43:59 EST 2000


A paper I will be presenting for a seminar course describes the in vitro
selection of specific scFv libraries that target an antigen of interest.
 This is done, as I understand it, by panning the scFv fragments bound
to phage against immobilized antigen.  However, how much use are the
resulting specific scFv fragments alone for antibody applications?  Are
these scFv genes typically used to create entire antibody fragments?  If
so, how is this done?


Josh Neufeld
PhD candidate
Department of Microbiology and Immunology
University of British Columbia

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