radioisotopes vs. chemilumenesence

Chris Fields cjfields at jove.acs.unt.edu
Sun Oct 1 22:48:26 EST 2000


I would like the protocol too.

Thanks!

Arnoud van Vliet wrote:
> 
> Hi Paul
> 
> I have been working a lot on getting DIG-Northerns to work on bacterial RNA,
> and I have it working now (most of the time). I can mail you a protocol
> (with some tips) if you like.
> 
> hope this helps
> Arnoud
> 
> "Paul Cullen" <Paul.Cullen at med.monash.edu.au> wrote in message
> news:39D1455C.42D8047E at med.monash.edu.au...
> > Adam,
> >      I've also been trying a non radioactive northern protocol - with no
> > joy yet. I'm using the DIG system and also have been unable to find a
> > comparison of the sensitivities.
> >
> > adam biales wrote:
> > >
> > > Does anyone know the comparitive sensitivities between radioisotopes and
> > > chemilumenessence or fluorometrics.  I am trying to justify using a
> > > nonradioactive northern protocol and I can't find information regarding
> > > relative sensitivities.
> > >
> > > Thanks,
> > > Adam Biales
> >
> > --
> > Paul Cullen
> > Bacterial Pathogenesis Research Group
> > Monash University
> > Australia

-- 
C. J. Fields
Graduate Student, Dept. of Biological Sciences
The University of North Texas
Denton, TX 

email : cjfields at jove.acs.unt.edu
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