Why do probes stick to RNA markers?

Susanne Rohrer "srohrer(removethis)" at immv.unizh.ch
Mon Oct 2 05:17:05 EST 2000



Pamela Norton wrote:

>
> I agree with Juergen's response, but in addition you need to consider
> that a low level of crosshybridization can have a major effect due to
> the high copy number of the RNA markers. However, it is puzzling that
> you have bands that don't correspond to the marker bands. Maybe you
> have a low level of contamination in your loading dyes or something?

These RNA markers are not very stable. So it may be degradation products of the
bands that crosshyb.







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