Stability of stored PCR Templates?
pmitsis at seqltd.com
Mon Oct 2 10:50:24 EST 2000
We've been running a series of PCR experiments on approximatelty 100 bp
DNAs with potentially a lot of secondary structure. Recently our stored
templates have become more and more refractory to re-amplification. These
are purified from PCR reactions (Qiagen) and stored at -20oC in TE. My
guess was aggregation but none of the simple things to try to dissagregate
them (heat or urea) seems to help. Has anyone seen this before or have a
suggestion what might be going on.
Thanks for any help you can give.
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