Stability of stored PCR Templates?

Paul Mitsis pmitsis at seqltd.com
Mon Oct 2 10:50:24 EST 2000


Hi All,

    We've been running a series of PCR experiments on  approximatelty 100 bp
DNAs with potentially a lot of secondary structure. Recently our stored
templates have become more and more refractory to re-amplification.  These
are purified from PCR reactions (Qiagen) and stored at -20oC in TE.  My
guess was aggregation but none  of the simple things to try to dissagregate
them (heat or urea) seems to help.  Has anyone seen this before or have a
suggestion what might be going on.
Thanks for any help you can give.

Paul Mitsis
Senior Scientist
Praelux Inc.








More information about the Methods mailing list