liquid or semidry western blotting?

Richard P. Grant rpg14 at
Tue Oct 3 10:46:07 EST 2000

In article <v04011700b5ff930e2a11@[]>, 
mlsulliv at ("Michael L. Sullivan") wrote:

> Now, as for the huge buffer tank, I've been using an old Hoeffer set up,
> which I think in theory should hold 5 l of buffer.  I've been making 4
> liters (1X tris-glycine, 20 % MeOH) and reusing it 4 times.  Reusing it
> doesn't seem to be a problem.  I just put it in a jug and put it in the
> cold room.

Can't you place something in the tank to displace some volume?  If one 
blots in the cold room, then cooling should not be affected.

Richard P. Grant MAD Phil 

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