liquid or semidry western blotting?
Richard P. Grant
rpg14 at yahoo.co.uk.invalid
Tue Oct 3 10:46:07 EST 2000
In article <email@example.com>,
mlsulliv at facstaff.wisc.edu ("Michael L. Sullivan") wrote:
> Now, as for the huge buffer tank, I've been using an old Hoeffer set up,
> which I think in theory should hold 5 l of buffer. I've been making 4
> liters (1X tris-glycine, 20 % MeOH) and reusing it 4 times. Reusing it
> doesn't seem to be a problem. I just put it in a jug and put it in the
> cold room.
Can't you place something in the tank to displace some volume? If one
blots in the cold room, then cooling should not be affected.
Richard P. Grant MAD Phil http://www2.mrc-lmb.cam.ac.uk/personal/rpg/
Please reply to rpg 'at' mrc-lmb.cam.ac.uk'
More information about the Methods