liquid or semidry western blotting?

Richard P. Grant rpg14 at yahoo.co.uk.invalid
Tue Oct 3 10:46:07 EST 2000


In article <v04011700b5ff930e2a11@[144.92.64.174]>, 
mlsulliv at facstaff.wisc.edu ("Michael L. Sullivan") wrote:

> Now, as for the huge buffer tank, I've been using an old Hoeffer set up,
> which I think in theory should hold 5 l of buffer.  I've been making 4
> liters (1X tris-glycine, 20 % MeOH) and reusing it 4 times.  Reusing it
> doesn't seem to be a problem.  I just put it in a jug and put it in the
> cold room.

Can't you place something in the tank to displace some volume?  If one 
blots in the cold room, then cooling should not be affected.

-- 
Richard P. Grant MAD Phil      http://www2.mrc-lmb.cam.ac.uk/personal/rpg/ 

Please reply to rpg 'at' mrc-lmb.cam.ac.uk'






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