Inclusion body

Aki Hoji akhst7 at imap.pitt.edu
Tue Oct 3 02:42:39 EST 2000


Dear, 

I tried to dissolve the purified inclusion body in the urea buffer (8M Urea,
25mM MES, 1mM DTT 0,5mM EDTA) at room temp. overnight on the rotor but it
did not.  I'd appreciate any suggestions or comments.  Thanks in advance.


regards, 



Aki Hoji
University of Pittsburgh
Department of Infectious Diseases and Microbiology
Rm522 Parran Hall, GSPH-IDM
130 Desoto Street, Pittsburgh PA 15261
tel: (412) 624-3072/0776
fax: (412) 624-4953






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