liquid or semidry western blotting?
"srohrer(removethis)" at immv.unizh.ch
Wed Oct 4 07:17:07 EST 2000
"Richard P. Grant" wrote:
> Not thought of that - but IIRC the BioRad kit has the electrodes on the
> walls, and the ice'cube' inbetween either electrode and the sandwich.
> I'd be terribly surprised if it made a noticeable difference. That's my
> gut instinct.
The BioRad apparatus has the membrane sandwiches inside a plastic thingy where
the electrode wires are, and the ice compartment outside of that. But since
the sandwiches are clamped inside a plastic grid with holes in it, apparently
there is also somehing "in the way" of the current (bad physics here, I know)
so it shouldn't matter. How about putting in some glass marbles?
More information about the Methods