genomic DNA PCR

Robert Mihalek rmihalek at
Wed Oct 4 07:59:33 EST 2000

Can someone forward me a general protocol for doing PCR using genomic
DNA as the template?  I seem to recall needing about 100 ng of DNA, but
I forget stuff like  how much Taq to use, concentration of dNTP's, and
concentration of primers.

Many thanks!

Bob Mihalek
Mass. General Hospital
Dept. of Anesthesia
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