genomic DNA PCR

Dr. Duncan Clark Duncan at
Wed Oct 4 10:06:58 EST 2000

In article <8rf9jl$n0h$1 at>, Robert Mihalek
<rmihalek at> writes
>Can someone forward me a general protocol for doing PCR using genomic
>DNA as the template?  I seem to recall needing about 100 ng of DNA, but
>I forget stuff like  how much Taq to use, concentration of dNTP's, and
>concentration of primers.

1.25u Taq per 50ul PCR (add last)
MgCl2 1.5mM
200uM dNTPs
0.5uM each primer
30ng human xsomal (10,000copies)
water to 50ul

The problem with being on the cutting edge is that you occasionally get 
sliced from time to time....

Duncan Clark
DNamp Ltd.
Tel: +44(0)1252376288
FAX: +44(0)8701640382

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