Hello. I am having trouble with my Pfu polymerase. Template is genomic DNA and the size of the product should be 2 and 3 kb. With the Taq polymerase I have no problem amplifying the sequence, also when I try smaller bits (like 500 bp) I do get a product with Pfu. But I can not Pfu amplify the large sequnces. Maybe it should be noted that the template i 80 % AT.