Pfu

Ali El-Salanti isl40117 at worldonline.dk
Thu Oct 5 03:25:36 EST 2000


Hello.
I am having trouble with my Pfu polymerase. Template is genomic DNA and the
size of the product should be 2 and 3 kb. With the Taq polymerase I have no
problem amplifying the sequence, also when I try smaller bits (like 500 bp)
I do get a product with Pfu. But I can not Pfu amplify the large sequnces.
Maybe it should be noted that the template i 80 % AT.








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