isl40117 at worldonline.dk
Thu Oct 5 04:58:25 EST 2000
The PCR machine is a PE. GeneAmp 2400.
The cycling goes as this:
1. 94, 5 min
2. 30 cycles of
94, 45 sec.
55, 45 sec.
72, 5 min
3. 72, 10 min
"Ali El-Salanti" <isl40117 at worldonline.dk> wrote in message
news:8rhe4v$qp0$1 at news.net.uni-c.dk...
> I am having trouble with my Pfu polymerase. Template is genomic DNA and
> size of the product should be 2 and 3 kb. With the Taq polymerase I have
> problem amplifying the sequence, also when I try smaller bits (like 500
> I do get a product with Pfu. But I can not Pfu amplify the large sequnces.
> Maybe it should be noted that the template i 80 % AT.
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